Publications

 

2013-present

 

  1. Hokkanen S., Laakso S., Senn C.M., Frey A.D. The trans-10,cis-12 conjugated linoleic acid increases triacylglycerol hydrolysis in yeast Saccharomyces cerevisiae. J Appl Microbiol. 2017 Mar 9. doi: 10.1111/jam.13443.
  2. de Ruijter J.C., Jurgens G., Frey A.D. Screening for novel genes of Saccharomyces cerevisiae involved in recombinant antibody production. FEMS Yeast Res. 2017 Jan;17(1). pii: fow104. doi: 10.1093/femsyr/fow104.
  3. de Ruijter J. C., Koskela E. V. and Frey, A. D. 2016. Enhancing antibody folding and secretion by tailoring the Saccharomyces cerevisiae endoplasmic reticulum. Microbial Cell Factories. 15:87.
  4. Piirainen M. A., Boer, H., De Ruijter J. C., and Frey A. D. 2016. A dual approach for improving homogeneity of a human-type N-glycan structure in Saccharomyces cerevisiae. Glycoconjugate Journal 33(2):189-99.
  5. Frey, A. D. and Aebi, M. 2015. An enzyme-based screening system for the rapid assessment of protein N-glycosylation efficiency in yeast. Glycobiology 25:252-257
  6. de Ruijter, J. C. and Frey, A. D. 2015 Analysis of antibody production in Saccharomyces cerevisiae: effects of ER protein quality control disruption. Appl Microbiol Biotechnol. 99(21):9061-71.
  7. Mueller, S., Wahlander, A., Selevsek, N., Otto, C., Ngwa, E. M., Poljak, K., Frey, A. D., Aebi, M., Gauss, R.  2015. Protein degradation corrects for imbalanced subunit stoichiometry in OST complex assembly. Molecular Biology of the Cell 26: 2596-2608.
  8. Koskela, E. V., Frey, A. D. 2015. Homologous Recombinatorial Cloning Without the Creation of Single-Stranded Ends: Exonuclease and Ligation-Independent Cloning (ELIC). Molecular Biotechnology 57:233-240
  9. Piirainen, M. A., de Ruijter, J. C., Koskela, E. V., Frey A. D. 2014. Glycoengineering of yeasts from the perspective of glycosylation efficiency. N Biotechnol. 31:532-7
  10. Nasab, F. P., Aebi, M., Bernhard, G., Frey A. D. 2013. A combined system to engineer glycosylation efficiency and glycan structure in Saccharomyces cerevisiae. Appl Environ Microbiol. 79:997-1007

 

Other

 

  1. Frey, A. D., Shepherd, M., Jokipii-Lukkari, S., Häggman, H. and P. T. Kallio. 2011. The single-domain globin of Vitreoscilla: Augmentation of aerobic metabolism for biotechnological applications. Advances in Microbial Physiology 58:81-139.
  2. Frey, A. D., Schmid, V. H. and P. T. Kallio. 2011. Genetic engineering of the pancreatic β-cell line MIN6 to express bacterial globin proteins protects cells from nitrosative stress. Biotechnology and Applied Biochemistry 58:84–90
  3. Karg S. R., Frey A. D., and P. T. Kallio. 2010. Reduction of N-linked xylose and fucose by expression of rat beta1,4-N-acetylglucosaminyltransferase III in tobacco BY-2 cells depends on Golgi enzyme localization domain and genetic elements used for expression. Journal of Biotechnology 146:54-65.
  4. Jokipii-Lukkari, S., Frey, A. D., Kallio, P. T., and H. Häggman. 2009. Intrinsic non-symbiotic and truncated haemoglobins and heterologous Vitreoscilla haemoglobin expression in plants. Journal of Experimental Botany 60:409-422.
  5. Frey, A. D., Karg, S. R., and P. T. Kallio. 2009. Expression of rat beta(1,4)-N-acetylglucosaminyltransferase III in Nicotiana tabacum remodels the plant-specific N-glycosylation. Plant Biotechnology Journal 7:33-48.
  6. Karg, S. R., Frey, A. D., Ferrara, C., Streich, D. K., Umaña, P., and P. T. Kallio. 2009. A small-scale method for the preparation of plant N-linked glycans for analysis by MALDI-TOF mass spectrometry. Plant Physiology and Biochemistry 47:160-166.
  7. Kallio, P. T., Bollinger, C. J. T., Koskenkorva, T., and A. D Frey. 2008. Assessment of biotechnologically relevant characteristics of heterologous hemoglobins in Escherichia coli. In: Globins and other nitric oxide-reactive proteins, Part A. Methods Enzymology 436C:255-72.
  8. Koskenkorva, T., Aro-Kärkkäinen, N., Bachmann, D., Arai, H., Frey, A. D., and P. T. Kallio. P. T. 2008. Transcriptional activity of Pseudomonas aeruginosa fhp promoter is dependent on two regulators in addition to FhpR. Archives of Microbiology 189: 385-396.
  9. Farrés, J., Burckhardt-Herold, S., Scherrer, J., Frey, A. D., and P. T.Kallio. 2007. Analysis of the contribution of the globin and reductase domains on the ligand binding properties of bacterial hemoglobins. Biochemical Journal. 407:15-22.
  10. Frey, A. D., Andersson, C. I. J., Schmid, V. H., Bülow, L., and P. T. Kallio. 2007. Globin-expression postpones onset of stationary phase specific gene expression in Escherichia coli. Journal of Biotechnology. 129:461-71.
  11. Fritzius, T., Frey, A. D., Schweneker, M., Meyer, D., and K. Mölling. 2007. WD-repeat-propeller-FYVE protein, ProF, binds VAMP2 and protein kinase C zeta. FEBS Journal 274:1552-66.
  12. Kallio, P. T., Heidrich, J., Koskenkorva, T., Bollinger, C. J. T., Farrés, J., and A. D. Frey. 2007. Analysis of novel hemoglobins during microaerobic growth of HMP-negative Escherichia coli. Enzyme and Microbial Technology 40:329-336.
  13. Fritzius, T., Burkard, G., Haas, E., Heinrich, J., Schweneker, M., Bosse M., Zimmermann, S., Frey, A. D., Caelers, A., Bachmann, A. S., and K. Mölling. 2006. A WD-FYVE protein binds to the kinases Akt and PKCzeta/lambda. Biochemical Journal 399:9-20.
  14. Ruohonen, L., Aristidou, A., Frey, A. D., Penttilä, M., and P. T. Kallio. 2006. Expression of Vitreoscilla hemoglobin improves the metabolism of xylose in recombinant yeast Saccharomyces cerevisiae under low oxygen conditions. Enzyme and Microbial Technology 39:6-14.
  15. Koskenkorva, T., Frey, A. D., and P. T. Kallio. 2006. Characterization of heterologous hemoglobin and flavohemoglobin promoter regulation in Escherichia coli. Journal of Biotechnology 122:161-75.
  16. Farrés, J., Rechsteiner, M. P., Herold, S., Frey, A. D., and P. T. Kallio. 2005. Ligand binding properties of bacterial hemoglobins and flavohemoglobins. Biochemistry 44:4125-34.
  17. Frey, A. D., and P. T. Kallio. 2005. Nitric oxide detoxification - a new era for bacterial globins in biotechnology? Trends in Biotechnology 23:69-73.
  18. Frey, A. D., Oberle, B. T., Farrés, J., and P. T. Kallio. 2004. Expression of Vitreoscilla haemoglobin in tobacco cell cultures relieves nitrosative stress in vivo and protects from NO in vitro. Plant Biotechnology Journal 2:221-231.
  19. Frey , A. D., Koskenkorva, T., and P. T. Kallio. 2003. Vitreoscilla hemoglobin promoter is not responsive to nitrosative and oxidative stress in Escherichia coli. FEMS Microbiology Letters 224:127-32.
  20. Frey, A. D., and P. T. Kallio. 2003. Bacterial hemoglobins and flavohemoglobins: versatile proteins and their impact on microbiology and biotechnology. FEMS Microbiology Reviews 27:525-545.
  21. Häggman, H., Frey, A. D., Aronen, T., Ryynänen, L., Julkunen-Tiitto, R., Timonen, H., Pihakaski-Maunsbach, K., Chen, X., and P. T. Kallio. 2003. Expression of Vitreoscilla hemoglobin in hybrid aspen (Populus tremula x tremuloides). Plant Biotechnology Journal 1:287-300.
  22. Frey, A. D., Farrés, J. Bollinger C. J. T., and P. T. Kallio. 2002. Bacterial hemoglobins and flavohemoglobins for alleviation of nitrosative stress in Escherichia coli. Applied and Environmental Microbiology 68:4835-4840.
  23. Kallio, P. T., Frey, A. D., and J. E. Bailey. 2001. From Vitreoscilla hemoglobin (VHb) to a novel class of growth stimulating hemoglobin proteins. In: Recombinant protein production with prokaryotic and eukaryotic cells. A comparative view on host physiology. Kleuwer Academics Publishers. pp. 75-87.
  24. Frey, A. D., Rimann, M., Bailey, J. E., Kallio, P. T., Thompson, C. J., and M. Fussenegger. 2001. Novel pristinamycin-responsive expression systems for plant cells. Biotechnology and Bioengineering 74:154-163.
  25. Frey, A. D., Fiaux, J., Szyperski, T., Wüthrich, K., Bailey, J. E., and P. T. Kallio. 2001. Dissection of central carbon metabolism of hemoglobin-expressing Escherichia coli by 13C nuclear magnetic resonance flux distribution analysis in microaerobic bioprocesses. Applied and Environmental Microbiology 67:680-687.
  26. Frey, A. D., Bailey, J. E., and P. T. Kallio. 2000. Expression of Alcaligenes eutrophus flavohemoprotein and engineered Vitreoscilla hemoglobin-reductase fusion protein for improved hypoxic growth of Escherichia coli. Applied and Environmental Microbiology 66:98-104.

 

Patents and invention disclosures

  1. Host cells and methods for the cytoprotection from oxidative and nitrosative stress. Frey Alexander Daniel, Kallio Pauli Teuvo. EP1767643 (A1).
  2. Novel tools for the production of glycosylated protein in host cells. Helenius Jonne, Neupert Christine, Aebi Markus, Parsaie Nasab Farnoush, Frey Alexander Daniel. WO 2010049177 (A1)
  3. Improved glycosylation of proteins in host cells. Aebi Markus, Parsaie Nasab Farnoush, Frey Alexander Daniel. WO 2011/134643 A1
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